Thymosin Beta 4: Everything You Need To Know

Thymosin Beta 4: Benefits, Uses, Dosage and More

Thymosin beta 4, also known as TB4 (tβ4) and TB-500, is a peptide therapy that can be used to help individuals overcome certain symptoms and health concerns. Peptide therapy has boomed over the past few years and there are now more peptides than ever being used for peptide therapies.

Thymosin beta 4 is a peptide that influences the entire system of the body and plays a major role in immunity and wound healing.

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Elite HRT

Written by Elite HRT
on June 09, 2021

Medically reviewed by

Camille Frecking, Regulatory Affairs Specialist, MEDICAL ADVISOR


Keep reading to learn more about what thymosin beta 4 is, how it works in the body, its benefits, side effects, dosage, recommendations, forms, and more. 

What is Peptide Therapy?

Because peptide therapy is newer, you may be unfamiliar with it. Peptide therapy overall is the use of peptides to improve certain symptoms. Peptides are built up of amino acids, the molecules that makeup proteins. 

These peptides can usually be broken up into human growth hormone (HGH) peptides and non-HGH peptides. The HGH peptides are those that help to increase the levels of growth hormone through a variety of mechanisms. Some of the most common HGH peptides are ipamorelin and sermorelin. 

Other non-HGH peptides are those that do not directly influence the boost of human growth hormone and instead act on the body in different ways. These peptides are usually specialized in their functions and can be picked and chosen based on the symptoms you are experiencing. For example, thymosin beta 4 is a non-HGH peptide that is used commonly for promoting tissue regeneration and tissue repair.

What is Thymosin Beta 4?

Thymosin beta 4 is a non-HGH peptide that is a small peptide, meaning it is made up of fewer amino acids than others. It is a peptide that is water-soluble and can be found in every cell in the body except for red blood cells. Because of its widespread nature you can see just how important the role of tβ4 is. 

How Does Thymosin Beta 4 Work in the Body?

Thymosin beta 4 is found naturally throughout the body, and its primary function is to help the body to heal. It is usually released by platelets, the clotting agents of the blood, as well as immune cells to protect the body cells from becoming more damaged, inflamed, or infected, especially when it comes to reducing cellular apoptosis (i.e. cell death). 

Specifically, it binds to the protein G actin and helps to move cells to where they need to be so that they can regenerate tissues and form new tissues that can help to heal the body. 

Thymosin beta 4 also plays the role of decreasing the number of cells that build between muscle cells and other fibers, which play a role in creating scars after a wound. Overall, thymosin beta 4 has a wide scope of things that it does in the body, and the thymosin beta 4 injections help to produce these same effects by boosting the amount of the peptide that is found in the body. 

What Are the Benefits of Thymosin Beta 4?

The benefits of thymosin beta 4 impact almost the entire body and its systems. There are a few main benefits that help to improve the way the body works so that you can look and feel your best. 

The first is that it boosts the immune system. Since thymosin beta 4 is vital in attaching to immune cells it plays a role in immunity and can help to protect the body when it is under attack. 

There are also many benefits related to the skin including boosted collagen formation, hair growth, and diminishing the growth of scar tissue. The role of thymosin beta 4 in reducing myofibroblasts helps to reduce scar tissue and reduce the look and growth of scars. It can also help to make hair feel fuller and healthier to give yourself a more polished and healthy look. 

Thymosin Beta 4 is also very important in repairing soft tissues such as tendons, ligaments, and muscles. By impacting the role of the protein actin in the body it can help to promote healing of fibrous brands in these body tissues and help to improve the recovery process. There are even more uses and benefits for thymosin beta 4, but these are the largest ones that most relate most directly to the body’s natural use of thymosin beta 4. 

Who Should Take Thymosin Beta 4?

Thymosin beta 4 is recommended for people who experience symptoms such as a weakened immune system, have soft tissue damage or are prone to injury, want improved hair and skin appearance, and who may want a reduced appearance to a scar that is forming. 

When talking with your doctor about your symptoms, if any of these are brought up, you could be a candidate for this peptide therapy. In addition, those that are aging may be experiencing these symptoms with the natural process of aging.

Keeping this in mind, if you are experiencing unwanted side effects of aging such as wrinkles or decreased immunity, thymosin beta 4 could be an option. Remember to only take thymosin beta 4 if prescribed by a healthcare provider.

What Are the Side Effects of Thymosin Beta 4?

There are some things to keep in mind when it comes to taking a new medication and side effects are one of them. 

Ignoring the side effects can be tempting so you can only focus on the positives, but being an informed consumer is important so that you can make sure you are making the most well-informed decision about your health. 

With that being said, the most common side effect of thymosin beta 4 is injection site pain and redness. This injection site reaction should diminish over a few hours to days as it begins to heal. 

Some people also may experience fatigue or headaches that usually diminish quickly. 

It is also important to know that thymosin beta 4 is not recommended for children. 

What is the Common Dosage of Thymosin Beta 4?

When it comes to choosing the right dose of thymosin beta 4 there is a safe range that your healthcare provider can stay in. 

The common safe dose range is 300 micrograms to 1 gram per day.

Whatever the dose is, it is most commonly given through a “break” from the medication, then continuing a cycle of 6 weeks on, 6 weeks off. This gives your body a break from the medication and keeps it working as well as possible in the body with the fewest side effects possible. 

It is always important to remember that doses will vary from person to person and you should always follow the direction of your healthcare provider. 

What Are the Forms of Thymosin Beta 4?

Subcutaneous injections of thymosin beta 4 are most commonly used, but there are some other forms of thymosin beta 4 as well. Since it can be used for a wide variety of functions, some formulations are better suited for these uses. 

For example, there is an eye drop form that helps with optic symptoms. There is also a topical gel that can help with symptoms on the skin surface. 

Each form is formulated specifically for that use and can help to control the symptoms of those areas of the body. So, the injectable is the most common form, but keep an open mind if you see an eye drop or topical gel out there as well. 


Thymosin beta 4 therapy is non-HGH peptide therapy that has systemic effects. Thymosin beta 4 has a high benefit to risk ratio with very few side effects present and many uses and benefits. Thymosin beta 4 is a great option for many people, so if you are experiencing any of the associated symptoms you should talk with your provider about if it could be helpful for you.

Elite HRT is an excellent resource that can guide you in the right direction with peptide therapy. After doing a thorough assessment of you and your medical history they can create a personalized care plan to help address as many of your needs as possible.


Thymosin beta 4 | A4M

Thymosin β4: a multi-functional regenerative peptide. Basic properties and clinical applications | PubMed

Thymosin Beta-4 – an overview | Science Direct